234 EV.E.I.L.S research program on infant skin from birth : Contribution to characterize different skin types

We conducted a research program focused on infant skin types (normal, dry, sensitive, atopic), using similar in vitro models and non-invasive clinical assessments. All infants had examination, barrier function hydration assessment. Other markers were selected depending types. In mostly based patented age-dependent reconstructed epidermis, customized for each problem, plus an innervated epidermis model. Skin surface is immature until 2 years (Confocal microscopy) dysbalanced (Raman spectroscopy) with high NMF content at birth rapid decrease till 6 months. Infants dry have low NMFs ceramides levels compared to adults. Sensitive children show of elevated inflammatory cytokines (children = adults). genetic risk atopy lower from Among them, the presence desquamation face redness neck folds may represent supplementary factor AD. Dry (dry atmosphere) provoked > adults) ceramides. (lactic acid applications) demonstrated elevation ILRA/IL1α. Th2 AD model increase CCL2, 5, 27 basal epidermal stem cells, both regulated by specific emollient. Staph aureus model, biofilm developed within 24 hours, prevented Depletion filaggrin (technology Small-hairpin RNA) induced filaggrin, expression keratohyalin granules numbers. Innervated showed that cell are more stimulated than adult conclusion, targeted noninvasive explorations useful study develop care products adapted their needs.